Denk, Winifried
<p>Jan 06, 2013 11:05 PM
German physicist;
directs Max Planck Inst in Heidelberg.
implemented 2 photon micro while a post-doc at MIT
image live cells deep in highly scattering tissures.
2nd invention: Serial block-face scanning EM
won Leibniz prize in 03 and Kavli in 2012.
good article at HHMI:
Janelia is in Virginia;
before him, biologists used confocal scope
key idea: dye molec absorbs 2 photons at same time.
probab of near simult hit by 2 phtons is low except near beam focus.
so, little or no excitation outside the focus.... no scattered light.
Note: early collab with post doc Rafa Yuste who was at David Tank's lab ...
imaged dendritic spines... measured how they accum calcium...
as a teen he took apart radios... natl extension of what I was doing with electronics as a kid.
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