Open in TheBrain

Qi, Stanley

<p>Mar 27, 2014 09:26 PM &nbsp;email is &nbsp;lei.qi@ucsf.edu</p>

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great talk today at Munzer Aud at Stfd on CRISPRi/ Cas9 in human cells. see below.</p>

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was a grad student at CAL; &nbsp;inter in methods of interrogating the kinetic coordination of

multiple genes during development and pathogenicity &nbsp;(via CRSPR).</p>

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see his lab > &nbsp;Research... exclt &nbsp;diagrams...</p>

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1) &nbsp;repurpose &nbsp;bacterial &nbsp;CRISPR system for controlling gene expression using&nbsp;

Watson-Crick basepairing RNAs.</p>

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we developed</p>

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1) &nbsp;CRISPRi &nbsp;= interference; programmable &nbsp;RNA-guided transcrip silencing of bacter, yeast and mammalian cells.</p>

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2) modular ways &nbsp;for endog gene activation and repression in mammalian cells</p>

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3) &nbsp;live imaging in mammalian cells using CRISPR</p>

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