New Biotech Makes It Much Easier to Genetically Modify Monkeys
<p>Mar 27, 2014 10:10 PM sci am.</p>
<p>
Cas = CRISPR associated proteins.</p>
<p>
The bacteria generate specific guide strands of RNA whenever they encounter a virus that is starting to hijack their cellular machinery. The <b>guide-RNA complements the viral DNA</b>, which is how the Cas proteins know where to cut. The bacteria then keep a copy of the viral DNA in their own genetic sequence between two CRISPR elements for future reference in case a similar virus tries to cause trouble later on.</p>
<p>
By developing strands of RNA that precisely complement the part of the DNA molecule that they want to change, investigators can steer the Cas proteins to a predesignated spot and cut out enough genetic material to permanently disrupt the usual expression of the DNA molecule at that location.</p>
<p>
g CRISPR works in macaques;</p>