Engineered Arteries
<p>Sep 30, 2015 08:29 PM</p>
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talk by Laura Niklason, Yale.</p>
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cells + elastin + collagen
collagen: confers the vast mech strength that arteries need.</p>
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started in mid 1990s (she was a post doc at MIT)</p>
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PGA... use as scaffold: seed with SMCs.</p>
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1999 in Science: bovine and porcine.</p>
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design requirements:</p>
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mechanics: must be stupendous... 3 billion times (pulses)</p>
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low thrombogenecity.</p>
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an autologous cells (otherwise immunogenic)</p>
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SMCs from old humans do not grow as well as young animals.</p>
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focused on mechanics: collagen is really important;
17% of dry weight is type 1 is collagen.</p>
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grow in young animal and wash the cells away.</p>
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first app: 6 mm: high blood flow; less clotting;</p>
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2004 spin out Humacyte ealry 2005;</p>
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42 cm in length and 6 mm in diam. grow in bioreactor.</p>
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decellularized....</p>
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burst strength > 3000 mm (like rupture str of native artery!</p>
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first app:</p>
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dialysis access: 419K patients....</p>
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most PTFE grafts fail at 12 months.</p>
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we tested initially in dogs and pigs but went to baboons (to avoid immunicity)</p>
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put into 14 baboons; and poke with needles.</p>
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83% patency rate</p>
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baboons pull out stitches....</p>
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no cells pre-implant....</p>
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at 6 months gets repopulated with SMCs.</p>
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hostperceives as native matrix. get regenerated host tissue.</p>
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we did three phase 1s in Poland total of 80 pateints</p>
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implanted in 2012.</p>
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poked 3X per week for dialysis.</p>
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used for two years! in dialyssi pts.</p>
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AHA Nov 2013...
100% patency! at 6 months.</p>
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no immune resoonse....</p>
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one liter per min flow rates.... v encouraging...</p>
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(she's worked on this for 20 years)... working for 2 years.</p>
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other conduits: trachea; they last for 2 months</p>
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(our lungs only last for 2 hours)</p>
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