Brain Mapping in High Resolution 2013

<p>Nov 24, 2013 10:17 PM</p>

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exclt article from Nature by Vivien Marx &nbsp;7 Nov 2013;

online at John's website. (to overcome small font,

go to bottom of document)

; &nbsp;cites Jeff Lichtman (Harvard)

central focus is the the new &nbsp;61 simult beam scanner from Zeiss...

work in collab with Winfied Denk at MPI in Heidelberg.

see figure page 150.</p>

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they will get it in about a year &nbsp;SEM from Zeiss.</p>

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Denk developed &nbsp;SBEM: serial block face EM; &nbsp;scan the uncut face!

(then cut it off) &nbsp;(then doesn't matter if slice is crumpled)</p>

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for mouse brain, needs diamond knife that is 10 mm wide...</p>

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large diamond! &nbsp;blade edge of 4 nm !!!!</p>

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image capture of whole mouse brain = 60 petabytes = 60K terabytes.</p>

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Lichtman: &nbsp;ATUM: &nbsp;automatic tape-collecting ultamictrotome.</p>

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cites Shawn Mikula in Denk's team; &nbsp;(post doc);

soak entire brain in osmium soln.</p>

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new instrument: &nbsp;3 T bytes per hour!</p>

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emits secondary electrons

detection requries 50 nsec. &nbsp;minimum.</p>

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1.2 Billion pixels each image.</p>

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Lich: &nbsp;with a 3d voxel that is 5nm on a side.

imaging a 1mm cube of brain = 8 petavoxels of data.</p>

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see Mortitz H quote (MPI)

city 10K X size of London: &nbsp;image every person, stairwell, etc and all communication.</p>

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