1. Studying Genes and Their Products

Genes Can Be Isolated by DNA Cloning

Restriction Endonucleases and DNA Ligases Yield Recombinant DNA

Cloning Vectors Allow Amplification of Inserted DNA Segments

Cloned Genes Can Be Expressed to Amplify Protein Production

Many Different Systems Are Used to Express Recombinant Proteins

Alteration of Cloned Genes Produces Altered Proteins

Terminal Tags Provide Handles for Affinity Purification

The Polymerase Chain Reaction Offers Many Options for Cloning Experiments

DNA Libraries Are Specialized Catalogs of Genetic Information