3. Enzyme Kinetics as an Approach to Understanding Mechanism

• Substrate Concentration Affects the Rate of Enzyme-Catalyzed Reactions

• The Relationship between Substrate Concentration and Reaction Rate Can Be Expressed with the Michaelis-Menten Equation

• Michaelis-Menten Kinetics Can Be Analyzed Quantitatively

• Kinetic Parameters Are Used to Compare Enzyme Activities

• Many Enzymes Catalyze Reactions with Two or More Substrates

• Enzyme Activity Depends on pH

• Pre–Steady State Kinetics Can Provide Evidence for Specific Reaction Steps

• Enzymes Are Subject to Reversible or Irreversible Inhibition

• BOX 6-1 Curing African Sleeping Sickness with a Biochemical Trojan Horse