NP - CPP Story overview
Skipped project
First draft of pictures see PDF
Story1
Figure - assembly of NP+RNA
see pdf
assembly on dsRNA and ssRNA for TL
Figure - delivery and stability of RNA
for inhibitors of the endocytotic pathway check: pmid: 14576351
positive control? DNA transfection?
tools
dsRNA for assembly
1-1000 of CoV2
1-3500 of CoV2
unspecific RNA 1000nt for example GFP
ssRNA for translation
has to be caped and polyadenylated
pseudourinylation to compare with vaccineRNA?
has to have cov-leader for correct NP assembly + GFP reporter
just S protein for vaccine comparison test - check protein production by Western
which constructs: XXXXXX
biontech vaccine
sequence and modifications XXXX?
testing in cell culture XXXX
collaborations
Ralf - RNA tools?
Harald - epithelial infection model??