RNA binding affinities - overview
Collect studies documenting RNA binding of N
CoV2
Fulllength N
EMSA
2021 - PMID: 34381460 / 7mer FAM-Oligo: ssRNA/dsRNA 0.5uM
Fluorescence polarisation
PMID: 32416961 / 14mer DNA probe with a fluorescence label - to bind the dsDNA - Kd value is 191 ± 0.036 nM.
CTD
MST
(Zinzula Baumeister BBRC 2020 PMID: 33039147) ca. 4 uM; Sequence: 5’-Cy5-AUUAAAG; measured by MST in MST buffer 0.05% Tween after min incubation
RBD
other CoVs
SARS-CoV
(Giedroc 2009 pmid: 19782089) The reported affinity of N for U20 is in the 1-10 μM range (Kd) with no evidence for or against RNA binding specificity.23
PMID: 34765991 - EMSA of different SARS-CoVs