NP - RNA binding characterisation

Overview

N has at least two RNA binding domains and the IDRs. Which of these domains does contribute to RNA binding.

Create Domain mutants - Cov2 Wuhan and Omicron; in future only Omicron variants.

Check for the phosphorylated and non phosphorylated forms.

published papers to cite (or not)

2021 fluorescence polarisation (pmid: 34095780)

Next steps

Finish measurements with ssRNA and dsRNA

Chose constructs for NMR analysis.

combine data with PS project

Associated Projects

search of the PS signal: Identification of the PS - project

assembly of N on RNA - N positioning: NP - viral chromatin assembly

Protein constructs

ask Sabrina for picture

RNA molecules used

RNA 8-14 (short dsRNA); RNA8 (short ssRNA); RNA4 (long ssRNA); RNA12 for interaction in nanoDSF

for sequences: RNA oligos - Nucleocapsid project

Binding affinity measurements

2023 - Jonas Abel, Michaela Aichinger, Isabell Reinberger, Pia Schraml / Bioanalytik Praktikum

GRPS Page for Praktikum: GRIPS - ChemikerInnen / GRIPS - Bioanalytik

Gernot work/Arbeitsgruppe/Praktikanten/Jonas Abel 11-22 +raw data

Gernot work/Arbeitsgruppe/Praktikanten/Michaela Aichinger 3-23

Gernot work/Arbeitsgruppe/Praktikanten/Isabell Reinberger 4-23

Gernot work/Arbeitsgruppe/Praktikanten/Pia Schraml 4-23

Results overview

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