Stable viral RNA elements in the cell

(pmid: 32446559) Characteristic RNA sequences within RNA virus genomes or RNAs transcribed from DNA virus genomes contribute toward this inhibition.

FATE-Seq: detected multiple virus-derived RNA sequences that stabilized GFP reporter RNA

these RNA sequences and their predicted secondary structures are highly conserved between SARS-CoV and the novel coronavirus, SARS-CoV-2

These sequences have the potential to enhance the stability of viral RNAgenomes, thereby augmenting viral replication efficiency and virulence

Method: (i) DNA sequences derived from genomes of RNA viruses and DNA viruses are fragmented in silico (viral sequences), (ii) a vector library is constructed by inserting these viral sequences downstream of the coding sequence (CDS) of a reporter gene in an expression vector (vector library), (iii) the resulting chimeric mRNAs are transcribed in vitro using the vector library as the template (mRNA library), (iv) the mRNA library is transfected into cells and later retrieved at a set time point, and (v) the remaining chimeric mRNAs in the cells are measured with the use of next-generation sequencing (NGS)