RBD - nanoDSF screens

Screen of PharmAI predicted binder

RBD interaction with RNA measured by nanoDSF - 24.11.2020 Gernot

worked out very well

RBD 5uM; RNA3 20uM

Tm RBD (green): 49.7°C / RBD+RNA (blue): 46.8°C (Ton 1°C higher) / RNA alone (red)

File: 201120 RBD titration - 2 RBD+RNA 201123 (in folder Julia Welte/Gernot Computer MST Facility Auswertungen)

26.11.2020 - first nanoDSF screen finished

nanoDSF screen: RBD (5uM) + Enamine compounds (100 uM) - (11-20; Gernot Längst)

about 15% hits; Tm reduced by ca. 0.5 to 1°C - (lets see what they do)

File: 201120 RBD titration - 3-15 (in folder Julia Welte/Gernot Computer MST Facility Auswertungen)

in total 53 Inhibitors were selected; 25 showed clear nanoDSF results; 11 had a strange fluorescence and further inhibitors were selected due to MST data

Example screenshot of one of the 13 nanoDSF runs

list of compounds see: List of RNA-binding inhibitors found