Buffers
<p><b>Tris-acetate-EDTA (TAE) </b>buffer is historically the most common buffer used for agarose gel electrophoresis in the analyses of DNA products resulting from PCR amplification, DNA purification protocols, or DNA cloning experiments. This buffer has a low ionic strength and low buffering capacity. It is best suited to electrophoresis of large (>20 kb) pieces of DNA and will need to be replaced frequently or recirculated for longer (>4 h) gel run times.
 
<b><i>TAE;</i></b><i> Prepare a 10x stock solution in 1 liter of H2O:
48.4 g Tris base [tris(hydroxymethyl)aminomethane] 11.4 ml glacial acetic acid (17.4 M)
20 ml of 0.5 M EDTA or 3.7 g EDTA, disodium salt.
Dissolve all in 800 ml deionized water and mass up to 1 liter, store in room temperature and the solution should be diluted to 1X prior to use [100 ml (10 x stock) up to 1 liter deionized water].</i></p>