Bromocresol Green

Bromocresol Green (BCG) is a triphenylmethane dye belonging to the sulfonephthalein class of dyes. It is widely used as a pH indicator, transitioning from yellow below a pH of 3.8 to blue above a pH of 5.4[3]. This property makes it valuable in various applications, including growth mediums for microorganisms, titrations, and as a diagnostic tool in clinical practice. One of its most common uses is in measuring serum albumin concentration within mammalian blood samples, which can indicate kidney failure or liver disease[3]. Additionally, BCG is utilized in thin-layer chromatography (TLC) staining solutions to visualize acidic compounds[3].

The chemical formula for Bromocresol Green is C21H14Br4O5S, and it has a molecular weight of approximately 698.01 Da[6]. It is soluble in sodium hydroxide solution, water (6 mg/ml), ethanol (40 mg/ml), benzene, and diethyl ether[9]. The dye is also used as a tracking dye for DNA agarose gel electrophoresis and in protein determinations[9][16].

Safety data sheets indicate that Bromocresol Green should be handled with care, wearing personal protective equipment to avoid contact with skin, eyes, or clothing, and ensuring adequate ventilation during use[2]. It is not considered hazardous by the 2012 OSHA Hazard Communication Standard[2], but it is advisable to treat all laboratory chemicals with caution[19].

For disposal, Bromocresol Green and its solutions must be disposed of in accordance with federal, state, and local environmental control regulations[11]. It is important to avoid release to the environment and to follow specific disposal instructions provided by manufacturers or regulatory authorities[20].

In summary, Bromocresol Green is a versatile dye used in various scientific and clinical applications, primarily as a pH indicator and for measuring serum albumin levels. Proper handling and disposal practices are essential to ensure safety and environmental protection.

Citations:

[1] https://pubchem.ncbi.nlm.nih.gov/compound/Bromocresol-green

[2] https://www.fishersci.com/store/msds?countryCode=US&language=en&partNumber=B3835&productDescription=BROMOCRESOL+GREEN+ACS+5G&vendorId=VN00033897

[3] https://en.wikipedia.org/wiki/Bromocresol_green

[4] https://www.sciencedirect.com/topics/pharmacology-toxicology-and-pharmaceutical-science/bromocresol-green

[5] https://www.carlroth.com/medias/SDB-9949-AU-EN.pdf?context=bWFzdGVyfHNlY3VyaXR5RGF0YXNoZWV0c3wyNjY1MDl8YXBwbGljYXRpb24vcGRmfHNlY3VyaXR5RGF0YXNoZWV0cy9oN2EvaDk2LzkwNTk5NTk2MDMyMzAucGRmfDFkZTNmNDRmMTVmNTI1NzMyYTQzNjIxODU4Zjk4MzcxNTgyNjAyOTIzNjJhYWZhMDJkMWEzOTg0ZGVkM2RhMmU

[6] http://www.chemspider.com/Chemical-Structure.6209.html

[7] https://www.thoughtco.com/how-to-make-bromcresol-green-indicator-608136

[8] https://www.carlroth.com/medias/SDB-T115-MT-EN.pdf?context=bWFzdGVyfHNlY3VyaXR5RGF0YXNoZWV0c3wyMjc0Mzd8YXBwbGljYXRpb24vcGRmfHNlY3VyaXR5RGF0YXNoZWV0cy9oMDIvaDBhLzkwNzA4MzM4NjA2MzgucGRmfGM3NjZiMzNiOTM0YzhmZTI3YWM0ZjEyNzEyYTU1ZTgzM2U2ZGY1NzkyNTc4OTUyZDQyOTM2MGM1ZWM5OTE3MTA

[9] https://www.scbt.com/p/bromocresol-green-76-60-8

[10] https://www.sciencedirect.com/topics/nursing-and-health-professions/bromocresol-green

[11] https://www.jscc.edu/about-jackson-state/administration/safety-and-security/sds-files/bromocresol-green.pdf

[12] https://www.chemicalbook.com/ChemicalProductProperty_EN_CB9447060.htm

[13] https://www.sigmaaldrich.com/US/en/product/sial/114359

[14] https://smile.oregonstate.edu/sites/smile.oregonstate.edu/files/bromocresol_green_msds.pdf

[15] https://pubchem.ncbi.nlm.nih.gov/compound/Bromocresol-green-_sodium-salt

[16] https://www.mpbio.com/us/bromocresol-green-5-g

[17] https://www.lobachemie.com/lab-chemical-msds/MSDS-BROMOCRESOL-GREEN-SOLUTION-CASNO-76-60-02271-EN.aspx

[18] https://byjus.com/question-answer/what-is-bromocresol-green/

[19] https://www.flinnsci.com/sds_134-bromcresol-green/sds_134/

[20] https://datasheets.scbt.com/sc-206048.pdf