Microscopy: Two Photon Microscopy (Kurt Thorn)
<p>Feb 25, 2018 08:51 PM</p>
<p>
best tutorial on 2PM = 2 photon microscopy.</p>
<p>
can start at min 11 up to minute 20or so. scan laser point by point in a raster.</p>
<p>
just collect all the light in a bucket... it is guaranteed to have come from one single spot
(where the two beams met and then emitted a photon.</p>
<p>
record with PMT = photomultiplier tubes.</p>
<p>
no pinhole (like a confocal);</p>
<p>
use pulsed laser but normal avg power v bright burst... only on abt 1 over 150k of the time.
(don't want to burn holes in sample or to need a power plant)
the laser itself hits the sample with two photons
titanijm sapphire laser.</p>
<p>
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