Two-photon excitation microscopy
<p>Apr 28, 2011 09:32 PM pioneered by Winifred Denk patented by him and lic. to Carl Zeiss.</p>
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allows micro of living tissue with fluor up to depth of about 1 mm.</p>
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two photons of comparably lower energy than needed for one photon excitation can also excit
a fluorophore in one quantum event.</p>
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because it requires confluence of two laser beams, so excitation is restricted to a tiny focal volume
like 1 femtoliter. so excitation is highly localized.</p>
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