Circuits YUSTE Rafael 2008
<p>Mar 15, 2011 09:18 PM
 
CIRCUIT NEUROSCIENCE...  linking function with the circuit micro-structure.
 
Cell types:  we cannot do this without a parts list.  we still do not know
the list of all the neuronal celll types.  anatomic,  e-phys and molecular.
 
transgenic animals are a huge step forward:  can be pre-labeled genetically.
 
he mentions:  Petilla interneuron nomenclature meeting
 
we need a parts list for most regions of the CNS.
 
Circuit Connectivity:  Cajal  "  most regions are the "impenetrable jungle where many
investigators have lost themselves"
 
circuit algorithms:  completely unknown  /  total ignorance.
 
circuit dynamics probelm:  neural circuits in all species have a very rich "inner life"
manifested by a high degree of organized spontaneous activity.
 
Methodological Problems:
 
Optical tools:  (here he's talking about  calcium imaging...  ie imaging firing neurons);
 
he says "single most important tech challenge in neurosci" is imaging voltage in neuro circuits with
single cell resolution.  need novel molecular sensors of voltage.
 
manipulating neuronal activity:  optogenetic methods...  need better ones.
 
visualizing synaptic connex:  glutamate photo-stimulation, viral approaches...
 
computation and theoretical methods:  we still have not develped the correct math formalisms
 
 
 
 
 
 
 
 
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