Sinha, Supriyo

<p>Nov 19, 2013 04:20 PM</p>

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Senior Res. Sci.</p>

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PhD 2007 from Stfd &nbsp;with Byer group.

he is a laser specialist.</p>

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stunning project! &nbsp;image &nbsp;100 flies simultaneously in vivo &nbsp;during behavior

use robotic fly handling; &nbsp;robo-surgery to remove cuticle (skull);

put all 100 on single optical table.

automated &nbsp;x y z &nbsp;scan &nbsp;(x y with galvos) and z by piezo.</p>

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see under project: &nbsp;Massively parallel brain imaging in live fruit flies:

rasies data acquisition by factor of 100;</p>

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hi thruput has already affected genomics/ proteinomics but not neuro.</p>

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here we construct instrument to image 100 alert flies to be imaged simult!! by 2 photon micro.

Drosoph: &nbsp;small brain, &nbsp;sophist behavior, &nbsp;Large # of strains with genetic alterations of brain,

utility of fluor imaging in flies.</p>

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Why massive parallel imaging: &nbsp;1) track neural dynamics across large # of flies with genetic CNS tweaks

to see what is broken/ preserved, 2) fly is model for devel disorders, neurodeg diseases and addiction

3) &nbsp;drug screening: &nbsp;allow cell effects of new cmpds to be ev al &nbsp;

4)do tim-laose imaging during matruation of fly embryos.

also use it for nematodes and zebrafish.</p>