PfSnf2Lcore aa286-843

PfSnf2Lcore aa286-843

His tagged baculo virus construct. Our pfSnf2Lcore used for screening

His6 tag

Length: 598 aa / Molecular weight: 69.987 kDa

Isoelectric point: 9.21 / Charge at pH 7: 12.28

Extinction Coefficient: 66,155 / A[280] of 1 mg/ml: 0.95 AU

6 Trp

Infos to PfSnf2L: PfSnf2L

AF3 modeling of the protein

PfSnf2Lcore286-850 (6-24)

https://golgi.sandbox.google.com/fold/56c5b9c76ce5a4f7

• downloaded
• PDB made with Chimera 1.8 for 1 of the 5 predicted (.pdb file above)
• all 5 predicted structure overlay saved as chimera 1.8 session (.cxs file above)
• files saved locally: /Users/gernot/Dropbox/Gernot work/Projects/Malaria/pfSNF2L/ATPase Alphafold predictions/Alphafold3/AF3 ATPasecore structures

Docking NH125 - AF3 model with SwissDock

PfSnf2Lcore286-843 NH125 AF3 SwissDock - Best binder is Pf!!

Next steps

Identify interacting region (PfSnf2Lcore286-843 NH125 AF3 SwissDock) - make mutations and check binding again

Structure +/-Inhibitor

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