PvSnf2Lcore aa169-850
Creating the ATPase core
starting from PvSnf2L - full length protein: PVP01_0905000 PlasmoDB / UniProt (VUZ95704)
extract ATPase sequences between the disordered domains for IDT order
sequence between aa169-850, optimized for spodoptera with geneart.
Vector: pFastBacHTA cloned with EheI-HindIII, additional stop at the end, N-His6+TEV
PfSnf2Lcore246-843 vs PvSnf2Lcore169-850
Identical Sites: 551 (92.1%)
Pairwise Identity: 92.1%
ordered construct at thermo: PvSnf2Lcore - baculo expression construct
Interaction with NH125
only weak >20uM; Watzlowik paper
sequence modeled with AF3
PvSnf2lcore169-850 (6-24)
https://golgi.sandbox.google.com/fold/2dbf6dca9852a30c
- downloaded
- PDB made with Chimera 1.8 for 1 of the 5 predicted (.pdb file above)
- all 5 predicted structure overlay saved as chimera 1.8 session (.cxs file above)
- files saved locally: /Users/gernot/Dropbox/Gernot work/Projects/Malaria/pfSNF2L/ATPase Alphafold predictions/Alphafold3/AF3 ATPasecore structures