kinetics of Transcriptome changes - SARS-CoV2

(pmid: 34106002) timescaled, gene-comparable transcriptome of SARS-CoV-2

During infection, viral transcription, rather than genome replication, dominates all viral RNA synthesis activities.

The most highly expressed viral gene is the nucleocapsid gene, followed by ORF7 and ORF3 genes, while the envelope gene shows the lowest expression.

Host transcription dysregulation keeps exacerbating after viral RNA synthesis reaches a maximum.

The most enriched host pathways are metabolism related.

timepoints (0, 3, 6, 12, 24, 48, and 72 hours postinfection [hpi]), viral RNA copies in the supernatant were quantified by qRT-PCR, and cellular RNAs from both the virus and host were analyzed.