NP - Dynamic Light Scattering - DLS/Panta
Aims
DLS detects the multimeric state of NP.
Can we destroy the dimerisation of NP and therefore also virus genome packaging?
Core Information to NP
How much protein is required for detection:
Which capillaries to use:
At which conditions do we see the formation of monomers:
detergent
diameter 19nm; Ex300/NP40; date 18-5-20
salt
800mM salt to disrupt
RNA binding of NP
did not work yet
latest protein fraction 117a; pooled 500ul aliquots
concentration: 20uM
PBS/0.5Pluronic - diameter 22nm; measured with standard capillaries
