Plasmodium berghei

PbSnf2L closest to PfSnf2L

(68.2% aa identity to PfSnf2L over a 1,348-residue local alignment; the next-best of the ten P. berghei PF00176 proteins reaches only 34%)

PBANKA_0942700 PlasmoDB PlasmoDB

A0A509AKD2 · A0A509AKD2_PLABA Uniprot: UniProt

Essential in the asexual blood stage, RGR 0.245 (CI −0.296 to 0.787, 2 measurements, PbGEM-292328) — Bushell et al. 2017, PMID 28708996.

Essential in liver stages? The genome-scale transmission/liver screen (Stanway et al. 2019, PMID 31730853) put 1,355 barcoded mutants through blood → midgut → salivary gland → liver → blood; that set is 889 dispensable, 440 slow, 21 essential, 7 insufficient-data, 2 fast, and PBANKA_0942700 is not in it. Its only appearance in those supplements is as one of fourteen generic ATPases in a metabolic-model reaction association.

Length (mean): 1,378 aa (1,378 codons)

Sequences: 2

Identical Sites: 919 (68.0%)

Pairwise Identity: 68.0%

Designing the ATPase core

Expression construct of ATPase core: PbSnf2Lcore146-773 - baculo expression construct