PbSnf2Lcore146-773 - baculo expression construct

Plasmodium berghei

Starting point - closest to PfSnf2L

PBANKA_0942700 PlasmoDB PlasmoDB

A0A509AKD2 · A0A509AKD2_PLABA Uniprot: UniProt

Checking for ATPase domain

region of pfSnf2L ATPase core -> extended to homologous region with PfSnf2L -> PbSnf2Lcore aa146-773 A0A509AKD2_PLABA

Length (mean): 599 aa (599 codons)

Identical Sites: 549 (91.5%)

Pairwise Identity: 91.5%

Gene synthesis with geneart

sequence between aa146-773, optimized for spodoptera with geneart.

Vector: pFastBacHTA cloned with EheI-HindIII, additional stop at the end, N-His6+TEV

23-8-23 ordered/finished Geneart: C-term His6

Project: PbSnf2Lcore / 23ABXWFD / 8/24/2023

see geneious - Plasmodium berghei folder in Malaria Folder

Length: 637 aa (638 codons)

Molecular weight: 74.861 kDa

Design details: Plasmodium berghei